Fig 1: Gas and Gai are direct substrates of DHHC5. Palmitoylation of recombinant Gas (A), Gai (B), and GAPDH (C) in vitro with mock immunoprecipitation (-), catalytically inactive form of DHHC5 (DHHS5), and DHHC5 are shown. As a negative control, HA was added to cleave all thioester bonds. DHHC total input as determined by Western blotting is shown below the palmitoylation panels.
Supplier Page from Abcam for Recombinant Human GAPDH protein